George Spracklin

This study uses tunable DNMT1 degradation to investigate DNA methylation dynamics and genome organization, revealing cooperative roles of DNMT1 and DNMT3B and their impact on heterochromatin and cell fitness.

The paper identifies three inactive chromatin states in HCT116 cells and shows how H3K9me3-HP1/β heterochromatin influences genome compartmentalization and loop extrusion barriers through DNA methylation.

The paper introduces UniNicE-seq, a method for high-resolution accessible chromatin profiling in formaldehyde-fixed and FFPE tissues without optimization steps.

This study identifies ZNFX-1 and WAGO-4 as key proteins for RNA-directed transgenerational epigenetic inheritance in C. elegans, showing their localization to liquid droplet structures and their role in maintaining siRNA expression across generations.