Daniele Fachinetti

Rearrangement of chromosome spatial organization in mouse zygotes using optical tweezers does not affect preimplantation development

Elevated MPS1 converts the fibrous corona into a source of chromosomal instability in colon cancer

This study shows that elevated MPS1 causes chromosomal instability in colon cancer by converting the fibrous corona into a source of merotelic kinetochore-microtubule attachments.

Functional CENP-B boxes are selectively conserved at centromeric regions

The paper identifies that canonical CENP-B boxes are highly conserved in centromeres but not in ectopic sites, showing selective conservation and functional binding.

Oxidative DNA lesions destabilize centromeres and drive chromosome instability

This study shows oxidative DNA damage destabilizes centromeres, leading to chromosome instability and mitotic defects in pre-tumorigenic cells.

Heterochromatin boundaries maintain centromere position, size and number

The paper investigates how heterochromatin boundaries maintain centromere position, size, and number through specific histone modifications and methyltransferases.

The Arp2/3 complex maintains genome integrity and survival of epidermal Langerhans cells

This study shows that the Arp2/3 complex is essential for genome integrity and survival of epidermal Langerhans cells, with Arpc4 deficiency leading to DNA damage and premature aging-like decline.

Non-canonical functions of UHRF1 maintain DNA methylation homeostasis in cancer cells

This study shows UHRF1 has non-canonical roles beyond DNMT1 activation in maintaining DNA methylation homeostasis in cancer cells.

Tunable DNMT1 degradation reveals cooperation of DNMT1 and DNMT3B in regulating DNA methylation dynamics and genome organization

This study uses tunable DNMT1 degradation to investigate DNA methylation dynamics and genome organization, revealing cooperative roles of DNMT1 and DNMT3B and their impact on heterochromatin and cell fitness.

Kronos scRT: a uniform framework for single-cell replication timing analysis

Kronos scRT enables single-cell replication timing analysis without cell sorting, analyzing thousands of cells from mouse and human cell lines.

A method to enrich and purify centromeric DNA from human cells

The paper develops a method to enrich and purify centromeric DNA from human cells, achieving 25-fold enrichment and up to 60% centromeric content in sequenced fractions.