Sriharsa Pradhan

This study identifies HIRA SUMOylation as a cause of chromatin instability in aging oocytes, leading to reduced fertility, and shows similar defects in human oocytes.

The paper investigates how HDAC and LSD1 inhibition alters chromatin accessibility and nucleosome positioning in cancer cells, revealing a JunB-mediated mechanism that highlights therapeutic vulnerabilities.

The paper shows that PIWI protein-dependent DNA N6-adenine methylation protects genomic sequences from deletion in Oxytricha during nuclear differentiation.

The paper shows RNA Pol I inhibition disrupts nucleolar structure, alters NPM1 localization, and changes chromatin landscape through histone modifications and DNA methylation.

The paper uses NEED-seq to show lamin B1 and B2 associate with distinct heterochromatin states and are linked to neuronal development.

The paper identifies three inactive chromatin states in HCT116 cells and shows how H3K9me3-HP1/β heterochromatin influences genome compartmentalization and loop extrusion barriers through DNA methylation.

The paper shows that promoter-adjacent DNA hypermethylation in TBX15 reduces gene expression in myoblasts and skeletal muscle without silencing it, suggesting a regulatory role in preventing overexpression.

This study shows that PARP1 poly ADP-ribosylates SET8, impairing its function and leading to altered H4K20 methylation domains and chromatin states.

EM-seq detects DNA methylation at single base resolution using less DNA than bisulfite sequencing, showing improved accuracy and reliability.

This paper introduces LR-EM-seq, an enzymatic deamination method for long-range methylation profiling of 5-mC and 5-hmC, achieving better-defined DMRs over 5 kb.

The paper introduces UniNicE-seq, a method for high-resolution accessible chromatin profiling in formaldehyde-fixed and FFPE tissues without optimization steps.

The paper uses NicE-viewSeq to study chromatin accessibility dynamics during the cell cycle and after histone deacetylase inhibition, finding preserved accessibility with subtle changes and correlations with gene expression.

The paper examines DNA hypermethylation in developmental contexts, identifying gene-specific methylation patterns and their links to transcriptional regulation in human cell types.