Stéphane Canaan

This paper develops CRISPRi-based inducible systems to fine-tune antibiotic susceptibility in Mycobacterium tuberculosis by targeting key genes involved in drug activation.

This paper describes a CRISPRi-based protocol to assess gene silencing and envelope transitions in Mycobacterium smegmatis as a model for NTM pathogenesis.

This study investigates the mechanism of action of pyrazinamide (PZA) in Mycobacterium tuberculosis, showing it kills bacteria by acidifying cytosol via pH-driven weak-acid permeation, independent of PanD and pantothenate levels.

The paper evaluates the antimycobacterial activity of salicylic acid derivatives and shows they disrupt intrabacterial pH homeostasis in M. tuberculosis.

The paper develops and validates fluorescence-based CRISPRi tools for gene repression and live single-cell imaging in mycobacteria.

This study identifies early life thermosensory impairments in Magel2-deficient mice and shows oxytocin treatment can rescue these deficits.