Pyrazinamide kills Mycobacterium tuberculosis via pH-driven weak-acid permeation and cytosolic acidification
Janïs Laudouze, Tatyana I. Rokitskaya, Akira Abolet, Vanessa Point, Alexander M. Firsov, Ljudmila S. Khailova, Maria Sofia Deschutter, Gabriela Gago, Jean‐François Cavalier, Stéphane Canaan, Alain R. Baulard, Yuri N. Antonenko, Alexandre Gouzy, Pierre Santucci
This study investigates the mechanism of action of pyrazinamide (PZA) in Mycobacterium tuberculosis, showing it kills bacteria by acidifying cytosol via pH-driven weak-acid permeation, independent of PanD and pantothenate levels.
bioRxiv · Sep 26, 2025 · PreprintMicrobiology