Rouslan G. Efremov

MISO: Microfluidic protein isolation enables single particle cryo-EM structure determination from a single cell colony

The paper introduces MISO, a method combining microfluidics and cryo-EM to determine protein structures from single cell colonies with high sensitivity.

Lipids modulate open probability of RyR1 under cryo-EM conditions

This study shows that lipid concentration affects RyR1 open probability under cryo-EM conditions, with higher concentrations increasing open probability but lipid nanodiscs keeping RyR1 closed.

Rapid small-scale nanobody-assisted purification of ryanodine receptors for cryo-EM

This paper describes a method to rapidly purify ryanodine receptors from small protein samples for cryo-EM, demonstrating its effectiveness by solving receptor structures.

Metamorphism of catalytic domain controls transposition in Tn3 family transposases

The paper describes cryo-EM structures of Tn3 family transposase TnpA, revealing a unique catalytic domain refolding mechanism that regulates transposition and target immunity.