Jan Steyaert

This study develops nanobodies to selectively probe different states of GBP coat assembly and function in human cells.

This study characterizes the structure and function of CCHFV RNA-dependent RNA polymerase, revealing its unique features and antiviral susceptibility.

The paper describes the structure and function of a membrane-spanning E3 ubiquitin ligase complex, revealing its role in developmental processes and linking mutations to human birth defects.

This study shows dimeric Alix nucleates CHMP4 polymerization, essential for ESCRT-III membrane remodeling, and demonstrates that dimerization is required for plasma membrane repair.

This paper describes a nanobody-based method for targeting diverse Acinetobacter baumannii isolates and related species, demonstrating its effectiveness in capturing bacteria from complex mixtures.

This study reports crystal structures of the QacA efflux pump in different conformational states, revealing structural dynamics and mechanisms of multidrug transport and extrusion.

The paper reports the cryo-EM structure of Pseudomonas aeruginosa LysP bound to lysine and a nanobody, revealing its transport mechanism and inhibition by L-4-thialysine.

The paper shows how Ten4 switches between self-recognition and Latrophilin binding to guide neuronal migration through exclusive structural interactions.

This study identifies nanobodies that modulate Rel and RelA enzyme activity by trapping them in specific conformational states, inhibiting (p)ppGpp synthesis and affecting bacterial virulence.

This study identifies nanobodies that act as molecular chaperones for glucocerebrosidase, improving its activity and stability, with potential therapeutic applications for Gaucher and Parkinson's diseases.

This study determines the structures of native SV2A, revealing its binding modes for tetanus neurotoxin and anti-epileptic drugs, and provides insights into toxin-receptor interactions and drug specificity.

This study identifies a conserved lysine in TM5 of BasC as critical for cytoplasmic gate dynamics, showing its role in gating transitions and modulation by substrates and mutations.

This paper introduces a biosensor-based phage display method for high-throughput Nanobody discovery, reducing time and labor by using automated Octet biolayer interferometry.

This paper describes a nanobody antagonist of the μ-opioid receptor, revealing its structural basis and designing peptide analogues with antagonistic activity.

This paper describes a method to rapidly purify ryanodine receptors from small protein samples for cryo-EM, demonstrating its effectiveness by solving receptor structures.

This study identifies and characterizes nanobodies that act as potent positive allosteric modulators of the calcium-sensing receptor, offering new tools for research and potential therapeutic applications.

This study determines the cryo-EM structures of the human thiamine transporter SLC19A3 in complex with ligands, revealing its transport mechanism and drug interactions.

This study determines the structure and dynamics of a Na+-coupled sugar transporter, revealing how mobile barriers regulate substrate binding and release.

The paper develops a synthetic Fab module (NabFab) that binds nanobodies with high affinity, enabling high-resolution cryo-EM studies of membrane proteins.

This study identifies a topological switch in CFTR's NBD1 domain that regulates channel function and contributes to misfolding in F508del-CFTR.

The paper identifies a conserved vestibule binding site in pLGICs and shows nanobodies can act as allosteric modulators, expanding drug design possibilities.

The paper reports cryo-EM and crystal structures of GABAA receptors with ligands, revealing the molecular basis of BZD modulation.

This study develops a biosensor to map opioid receptor activation in neurons, showing drugs activate receptors in the Golgi and dendrites, while peptides activate them in the plasma membrane and endosomes.