Author

Steven De Gieter

Vrije Universiteit BrusselORCID 0000-0002-2776-3791Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Assay:cryo-EM· 2 Approach:experimental assay· 2 Organism:bacteria (other)· 1 Research Focus:cell type annotation· 1 Organism:human· 1 Research Focus:protein folding & dynamics· 1 Research Focus:protein structure prediction· 1

Frequent Co-Authors

Annelore StroobantsiD· 1 shared Binesh Shrestha· 1 shared Chris UlensiD· 1 shared Gangadhar EluruiD· 1 shared Jana KuschiD· 1 shared Janine Denise BrunneriD· 1 shared Jessica Matos Kleiz-FerreiraiD· 1 shared Marijke BramsiD· 1 shared

Activity by Year

2025-2025 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 20 mutual co-author links

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1 shared paperBiophysics

Annelore StroobantsORCID

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2 papers

Recent·Most cited

MISO: Microfluidic protein isolation enables single particle cryo-EM structure determination from a single cell colony

Gangadhar Eluru, Steven De Gieter, Stephan Schenck, Annelore Stroobants, Binesh Shrestha, Paul J. A. Erbel, Janine Denise Brunner, Rouslan G. Efremov

The paper introduces MISO, a method combining microfluidics and cryo-EM to determine protein structures from single cell colonies with high sensitivity.

Nature Methods · Nov 13, 2025Peer-reviewedBiophysics

Structural basis for gating inhibition by the cytoplasmic domain in HCN1 channels

Valentina Zorzini, Jessica Matos Kleiz-Ferreira, Marijke Brams, Rodrigo U. Gallardo, Steven De Gieter, Jana Kusch, Chris Ulens

This study uses cryo-EM to analyze HCN1 channel variants, revealing structural differences in cAMP sensitivity and auto-inhibition mechanisms between HCN1 and HCN2 isoforms.

bioRxiv · Oct 14, 2025 · PreprintBiochemistry