Tammy Kielian

This study shows that Eap proteins in S. aureus biofilms reduce porosity and prevent macrophage invasion, enhancing biofilm survival during prosthetic joint infections.

This study develops BaSSSh-seq, a bacterial single-cell RNA sequencing method, to analyze transcriptional heterogeneity and immune responses in biofilms.

This study shows that Staphylococcus aureus counters acetate-mediated inhibition of peptidoglycan cross-linking by maintaining high intracellular D-Ala levels through alanine racemase and limiting D-Ala flux via D-alanine aminotransferase.