This study analyzes the free energy and flexibility of autoinhibited human BRAF using molecular dynamics simulations based on a cryo-EM model to understand its activation mechanism.

Journal of Chemical Information and Modeling · Apr 21, 2026Peer-reviewedBiophysics

An In Vitro BRAF Activation Assay Elucidates Molecular Mechanisms Driving the Disassembly of the Autoinhibited BRAF State

Daniel A. Ritt, David Durrant, Matthew R. Drew, Suzanne I. Sandin, Juliana A. Martinez Fiesco, Xiao‐Hua Zhang, Fikret Aydin, Timothy S. Carpenter, Grace M. Scheidemantle, Robert A. D’Ippolito, Alexandria L. Sohn, Caroline J. DeHart, Rebika Shrestha, Kelly Snead, Jenna Hull, , Deborah K. Morrison

This study develops an in vitro BRAF activation assay to elucidate molecular mechanisms of BRAF disassembly and identifies RAS binding as critical for activation.

Proceedings of the National Academy of Sciences · Sep 15, 2025Peer-reviewedBiochemistry

Apolipoprotein interaction induces shape remodeling and lipid phase separation in giant unilamellar vesicles

Christopher F. Carnahan, Wei He, Tuğba Nur Öztürk, Yaqing Wang, Viviane N. Ngassam, Aleksandr Noy, Timothy S. Carpenter, John C. Voss, Matthew A. Coleman, Atul N. Parikh

This study investigates how ApoA-I interacts with lipid membranes to induce shape remodeling and lipid phase separation in GUVs, revealing key steps in lipoprotein assembly.

bioRxiv · Apr 10, 2025 · PreprintBiophysics