An In Vitro BRAF Activation Assay Elucidates Molecular Mechanisms Driving the Disassembly of the Autoinhibited BRAF State
Daniel A. Ritt, David Durrant, Matthew R. Drew, Suzanne I. Sandin, Juliana A. Martinez Fiesco, Xiao‐Hua Zhang, Fikret Aydin, Timothy S. Carpenter, Grace M. Scheidemantle, Robert A. D’Ippolito, Alexandria L. Sohn, Caroline J. DeHart, Rebika Shrestha, Kelly Snead, Jenna Hull, , Deborah K. Morrison
This study develops an in vitro BRAF activation assay to elucidate molecular mechanisms of BRAF disassembly and identifies RAS binding as critical for activation.
Proceedings of the National Academy of Sciences · Sep 15, 2025Peer-reviewedBiochemistry