Craig S. Pikaard

Epi-PoreC profiles chromatin conformation, DNA methylation, and chromatin accessibility simultaneously using nanopore sequencing on single DNA molecules.

The paper fills genome gaps in Arabidopsis NOR2 and NOR4 sequences, revealing their distinct gene subtype compositions and epigenetic regulation.

The paper shows that AGO4 slicing of 23 nt passenger strands and retention of sliced target RNAs enhances RNA-directed DNA methylation in plants.

The paper shows that Pol IV and RDR2 transcript features determine siRNA size, sequence, and strand polarity, enhancing DNA methylation coverage.

This paper reports the structure of Arabidopsis RDR2 and shows how it initiates transcription from RNA templates displaced from RNA-DNA hybrids.

The paper shows that the CTD of NRPD1 is critical for siRNA production and target locus silencing but not for DNA methylation, indicating distinct roles for Pol IV in RdDM.

The paper investigates the role of the Pol V CTD in RNA-directed DNA methylation, showing its necessity for in vivo function and its interaction with RRP6L1.

The paper shows that an epiallele causes hybrid incompatibility in Arabidopsis thaliana by silencing a gene copy, leading to lethal gene combinations in hybrids.