Joaquı́n Gutiérrez

This paper presents a platform for dynamic control of bacterial co-culture composition using optogenetic feedback and develops a framework for modeling synthetic genetic circuits in E. coli.

The paper characterizes a synthetic bacterial oscillator using CRISPRi and shows it produces robust oscillations and affects cellular growth.

This study identifies gene expression burden in mammalian cells and introduces miRNA-based circuits to mitigate it, improving synthetic circuit performance.