Benjamin E. Rubin

The paper demonstrates that half-match recombination, independent of target-site complementarity, drives bridge RNA-guided excision and off-target insertion in IS621 recombination.

The paper redefines IS110 as a dynamic platform capable of expanding into large elements that disseminate antimicrobial resistance.

The paper shows conjugative plasmids eliminate non-permissive bacterial recipients through lethal zygosis, enabling their own spread and improving gene editing efficiency with shielded transposon vectors.

This study identifies host factors that influence VchCAST activity and demonstrates that the λ-Red recombineering system significantly enhances its editing efficiency in E. coli and other bacteria.

OriGen generates and validates new plasmid origins of replication, demonstrating its ability to create functional sequences distinct from existing wild types.