Ed S. Lein

This paper describes an ex vivo platform for studying adult human neocortical cell types and circuits using acute and cultured brain slices, enabling molecular-genetic manipulation and neuronal activity monitoring.

The paper maps schizophrenia-associated genomic loci to specific brain cell types using single-cell RNA-sequencing data, finding consistent enrichment in pyramidal cells, medium spiny neurons, and interneurons.

The paper reviews how single cell RNA sequencing is identifying new human cell types and proposes a machine learning method to improve cell type definitions in the Cell Ontology.

The paper introduces STRT-seq-2i, a high-throughput single-cell RNA-seq platform, and demonstrates its application on fresh mouse and frozen human cortical samples.

SmartScope2 is an automated neuron reconstruction tool that reduces image data requirements and increases imaging speed for human and mouse cortical neurons.

NeuronStitcher is a software suite that stitches non-overlapping neuron fragments from 3D image sections to reconstruct large and complex neurons efficiently.