Scientific ReportsMolecular BiologyPeer-reviewed journal article
STRT-seq-2i: dual-index 5′ single cell and nucleus RNA-seq on an addressable microwell array
Hannah HochgerneriD, Peter LönnerbergiD, Rebecca D. HodgeiD, Jaromír MikešiD, Abeer Heskol, Hermann Hubschle, Philip Lin, Simone PicelliiD, Gioele La MannoiD, Michael RatziD, Jude Dunne, Syed Akhtar Husain, Ed S. LeiniD, Maithreyan SrinivasaniD, Amit ZeiseliD, Sten LinnarssoniD
Abstract
Single-cell RNA-seq has become routine for discovering cell types and revealing cellular diversity, but currently no high-throughput platform has been used successfully on archived human brain samples. We present STRT-seq-2i, an addressable 9600-microwell array platform, combining sampling by limiting dilution or FACS, with imaging and high throughput at competitive cost. We applied the platform to fresh single mouse cortical cells and to frozen post-mortem human cortical nuclei, matching the performance of a previous lower-throughput platform.
- organism: mouse
- organism: human
- assay: scRNA-seq
- research focus: benchmarking
- research focus: software / tool
- approach: experimental assay
- Citations
- 78
- FWCI
- 4.02
- Field percentile
- top 5%
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