Author

Kai Sheng

Scripps Research InstituteORCID 0000-0001-9394-2256Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Research Focus:cell type annotation· 3 Approach:experimental assay· 3 Organism:bacteria (other)· 2 Assay:structural biology· 2 Research Focus:drug response / drug discovery· 1 Organism:human· 1 Research Focus:protein folding & dynamics· 1 Assay:proteomics· 1

Frequent Co-Authors

James R. WilliamsoniD· 3 shared Dmitry LyumkisiD· 2 shared Sriram AiyeriD· 2 shared Xiyu DongiD· 2 shared Benjamin F. CravattiD· 1 shared Bruno N. MelilloiD· 1 shared David R. LiuiD· 1 shared Evert NjomeniD· 1 shared

Activity by Year

2024-2026 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 27 mutual co-author links

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3 shared papersBiochemistry

James R. WilliamsonORCID

Mutual co-authors in cluster

Author profile →

3 papers

Recent·Most cited

ESCAPE: assigning site-specific activity to covalent ligands in cells by prime editing

Jason Tse, William R. Brothers, Rachel E. Hayward, Sabrina Barbas, Kai Sheng, Kathryn R. Spencer, Kristen E. DeMeester, Evert Njomen, James R. Williamson, David R. Liu, Bruno N. Melillo, Gene Wei-Ming Yeo, Benjamin F. Cravatt, Haoxin Li

ESCAPE identifies covalent ligand-protein interactions by using prime editing to disrupt cysteine residues and assesses their impact on cellular phenotypes.

bioRxiv · Jul 29, 2026 · PreprintBiochemistry

Domain consolidation in Bacterial 50S assembly revealed by Anti-Sense Oligonucleotide Probing

Kai Sheng, Xiyu Dong, Sriram Aiyer, Joan Lee, Selena Đorđević-Marquardt, Dmitry Lyumkis, James R. Williamson

The paper uses anti-sense oligonucleotides to disrupt RNA interactions in bacterial ribosome assembly, revealing intermediate structures and domain consolidation during RNP formation.

Nature Communications · Jul 18, 2025Peer-reviewedBiochemistry

Assembly of the Bacterial Ribosome with Circularly Permuted rRNA

Xiyu Dong, Kai Sheng, Luca F. R. Gebert, Sriram Aiyer, Ian J. MacRae, Dmitry Lyumkis, James R. Williamson

This study investigates bacterial ribosome assembly using circularly permuted rRNAs, revealing conserved assembly routes and domain association order.

Nucleic Acids Research · Jul 11, 2024Peer-reviewedBiophysics