Benjamin F. Cravatt

This study identifies a small molecule targeting PSME4, which stabilizes STAT1 and reprograms tumor inflammation, suppressing tumor growth by altering cytokine signaling.

ESCAPE identifies covalent ligand-protein interactions by using prime editing to disrupt cysteine residues and assesses their impact on cellular phenotypes.

This study identifies two mechanisms by which dipeptide esters disrupt lysosomal homeostasis: amyloid fibril formation and proximity labeling via Cathepsin C.

This study identifies erucamide as a key regulator of retinal neurovascular crosstalk, showing its role in stabilizing retinal degeneration through TMEM19-mediated activation of myeloid cells.

This study shows that inhibiting SagA, a peptidoglycan hydrolase, increases vancomycin susceptibility in VREfm through both genetic and pharmacological approaches.

The paper compares acrylamide and butynamide stereoprobes to identify covalent inhibitors of the actin maturation protease ACTMAP in human cancer cells.

This study uses a chemical degradation system to show FOXA1 opens chromatin exclusively at its binding sites, activating or repressing transcription based on the chromatin environment.

The paper identifies ortholog-restricted cysteines in Z. tritici that are targeted by stereoprobes, revealing potential fungicidal targets for combating wheat pathogens.

This study uses high-throughput chemistry to discover chemical inducers of proximity by modifying pre-existing ligands and screening for degrader activity.

The paper identifies host proteins regulating Mycobacterium tuberculosis replication in macrophages using cysteine reactivity profiling and genetic perturbation.

This paper reports covalent ligands targeting the RNA-binding protein NONO, showing they stabilize mRNA interactions, remodel transcriptomes, and suppress cancer cell growth with high specificity.

The paper develops covalent ligands that specifically target a complexoform of TRMT112, revealing partner-specific functional effects through allosteric activation of METTL5.

This paper develops vCATCH to map covalent drug targets across the entire mouse body, revealing new tissue and cell type engagements for afatinib and ibrutinib.

This study investigates the effects of ENL YEATS domain inhibitors in leukemia models, finding that suppression of ENL target genes is not sufficient to explain their anti-leukemia effects.

The paper identifies inhibitors of the GRPEL1-HSPA9 chaperone complex using tryptoline stereoprobes and shows they target non-orthosteric sites.

The paper constructs a genome-scale genetic interaction network from 4 million double mutants in HAP1 cells, revealing conserved genetic network principles across species and uncovering novel gene functions and cancer dependencies.

This study shows that PPARγ controls adipose tissue expansion by regulating LPCAT3, which affects membrane lipid composition and triglyceride storage, linking dietary n-6 PUFAs to energy balance.

The paper identifies LPCAT3 as a cold-regulated enzyme that generates AA-PE in BAT mitochondria, enhancing ETC efficiency and thermogenesis.

The paper uses stereoprobes to identify covalent ligands for proteins, revealing TLCD1 as a lipid acyltransferase inhibited by OTP stereoprobes.

This study identifies ophiobolin A's mechanism of action against EMT-positive breast cancer cells, showing mitochondrial and metabolic disruption with antioxidant countermechanisms.

This study validates the palmitoylation cycle as a therapeutic target in NRAS-mutant cancers by showing that inhibiting ABHD17 reduces tumor growth and synergizes with MEK inhibitors.

This paper shows that inhibiting ABHD11 reduces T cell cytokine production and alleviates autoimmunity by altering sterol metabolism and LXR activation.

The paper introduces photo-stereoprobes for expanding the ligandable proteome and demonstrates their use in discovering bioactive small molecules.

This study identifies covalent small molecules that bind FOXA1, altering its chromatin remodeling activity in prostate cancer cells.

The paper uses covalent probes and paralog hopping to identify ligandable pockets in proteins lacking cysteines, demonstrating a method to expand the ligandable proteome.

The paper uses base editing to assess the functional impact of cysteine mutations in cancer dependency genes, identifying essential and ligandable cysteines for drug development.

This study shows that DDHD2 and STXBP1 interact to generate myristic acid, which is essential for long-term memory formation in mice.

The paper uses scRNA-seq and SCOPE-seq to study adult neurogenesis, identifying NOTUM as a key regulator of V-SVZ NSC differentiation and neurogenesis.