Author

Nicholas W. Villarino

Scripps Research InstituteORCID 0000-0001-9608-2060Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Research Focus:cell type annotation· 2 Approach:experimental assay· 2 Research Focus:drug response / drug discovery· 1 Approach:genetic perturbation / CRISPR· 1 Organism:mouse· 1 Assay:proteomics· 1 Assay:spatial transcriptomics· 1

Frequent Co-Authors

Ardem PatapoutianiD· 2 shared Adrienne E. DubiniD· 1 shared Kara L. MarshalliD· 1 shared King L. HungiD· 1 shared Ryan A. PakiD· 1 shared Yu WangiD· 1 shared

Activity by Year

2022-2026 · peak 1/yr

Co-Author Collaboration Network

6 top collaborators · 9 mutual co-author links

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2 shared papersCell Biology

Ardem PatapoutianORCID

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2 papers

Recent·Most cited

Cell BiologybioRxivPreprintJul 3, 2026

A novel screening method using CRISPRa and FM 1-43 to identify cation channels

Ryan A. Pak, Nicholas W. Villarino, King L. Hung…·Ardem Patapoutian

The paper introduces a method combining CRISPRa and FM 1-43 to screen for cation channels by overexpressing targets and detecting activity with fluorescent labeling.

DOI 10.64898/2026.07.02.736146

proteomicsexperimental assay

𝕏 PostBluesky

NeurosciencebioRxivPreprintMay 26, 2022

Lighting Up Mechanosensation: dyeing to see PIEZO2

Nicholas W. Villarino, Adrienne E. Dubin, Ardem Patapoutian, Kara L. Marshall

The paper uses FM 1-43 dye to identify PIEZO2-expressing neurons involved in mechanosensation, revealing its role in urethral neurons during urination.

DOI 10.1101/2022.05.26.493600

mousespatial transcriptomics

𝕏 PostBluesky