Author

Ryan A. Pak

Scripps Research InstituteORCID 0000-0003-3507-3122Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Research Focus:cell type annotation· 1 Assay:CRISPR screen· 1 Research Focus:drug response / drug discovery· 1 Approach:experimental assay· 1 Approach:genetic perturbation / CRISPR· 1 Organism:human· 1 Research Focus:perturbation response prediction· 1 Assay:proteomics· 1

Frequent Co-Authors

Alina GunaiD· 1 shared Angela N. PogsoniD· 1 shared Ardem PatapoutianiD· 1 shared Baylee J. RusselliD· 1 shared Christina R. LiemiD· 1 shared Daniel M. RamosiD· 1 shared Jessica L. BonnariD· 1 shared Jonathan S. WeissmaniD· 1 shared

Activity by Year

2022-2026 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 29 mutual co-author links

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1 shared paperGenomics

Alina GunaORCID

Mutual co-authors in cluster

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2 papers

Recent·Most cited

Cell BiologybioRxivPreprintJul 3, 2026

A novel screening method using CRISPRa and FM 1-43 to identify cation channels

Ryan A. Pak, Nicholas W. Villarino, King L. Hung…·Ardem Patapoutian

The paper introduces a method combining CRISPRa and FM 1-43 to screen for cation channels by overexpressing targets and detecting activity with fluorescent labeling.

DOI 10.64898/2026.07.02.736146

proteomicsexperimental assay

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GenomicseLifePeer-reviewedDec 28, 2022

Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors

Joseph M. Replogle, Jessica L. Bonnar, Angela N. Pogson…·Marco Jost

This paper develops compact CRISPRi sgRNA libraries and optimal effectors to improve knockdown consistency and efficiency in mammalian cells.

152 citationstop 1%

humanCRISPR screen

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