Author

Ashley Hiett

University of Wisconsin–MadisonORCID 0000-0001-9891-7471Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Biophysics· 2

Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Assay:protein structure· 2 Organism:bacteria (other)· 1 Research Focus:protein folding & dynamics· 1 Research Focus:protein structure prediction· 1

Frequent Co-Authors

Benjamin D. HardingiD· 2 shared Chad M. RienstraiD· 2 shared Katherine A. Henzler‐WildmaniD· 2 shared Chao WuiD· 1 shared Claudia C. CornilescuiD· 1 shared Collin G. BorcikiD· 1 shared Eva-Maria UhlemanniD· 1 shared Grace E. Reichert· 1 shared

Activity by Year

2025-2026 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 39 mutual co-author links

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2 shared papersBiophysics

Benjamin D. HardingORCID

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2 papers

Recent·Most cited

BiophysicsbioRxivPreprintJun 1, 2026

Protonated Structure of EmrE Reveals C-terminal Tail Gating Mechanism

Ashley Hiett, Benjamin D. Harding, Merissa Brousseau…·Katherine A. Henzler‐Wildman

The paper reports the high-resolution structure of protonated EmrE, revealing how the C-terminal tail occludes the transport pore to prevent proton leak.

DOI 10.64898/2026.05.28.728512

protein structureexperimental assay

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BiophysicsBiomolecular NMR AssignmentsPeer-reviewedMay 26, 2025

Backbone and Sidechain 1H, 15N and 13C Resonance Assignments of a Multidrug Efflux Membrane Protein using Solution and Solid-State NMR

Benjamin D. Harding, Ashley Hiett, Marco Tonelli…·Katherine A. Henzler‐Wildman

The paper reports NMR resonance assignments for EmrE, a multidrug transporter, using solution and solid-state NMR techniques at specific pH conditions.

2 citations

bacteria (other)protein structure

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