Chad M. Rienstra

In vitro-prepared A30P alpha-synuclein fibrils adopt the conserved and disease-relevant Greek key fold

The paper reports the high-resolution SSNMR structure of A30P mutant alpha-synuclein fibrils, showing a conserved Greek key fold not including the mutation site, and validates a method for comparing SSNMR spectra of Asyn polymorphs.

Protonated Structure of EmrE Reveals C-terminal Tail Gating Mechanism

The paper reports the high-resolution structure of protonated EmrE, revealing how the C-terminal tail occludes the transport pore to prevent proton leak.

Top-Down Scoring of Spectral Fitness by Image Analysis for Protein Structure Validation

This paper introduces BPHON, a method that uses image analysis to score the fitness of protein structures against NMR spectra without requiring full resonance assignments.

NMR Spectral Alignment Utilizing a CryoEM Motion Correction Algorithm

This paper introduces ANSA, an automated NMR spectral alignment tool using cryo-EM motion correction, improving alignment accuracy and reducing manual effort in NMR data processing.

Selective Detection of a Key Region in Chemotaxis Signaling Protein Complexes by Solid-State NMR

This paper uses solid-state NMR to selectively detect rigid regions in chemotaxis signaling protein complexes, revealing conformational changes between signaling states.

Backbone Assignment of a 28.5 kDa Class A Extended Spectrum β-Lactamase by High-Field, Carbon-Detected Solid-State NMR

The paper reports high-resolution 13C and 15N backbone chemical shift assignments for Toho-1 β-lactamase using advanced solid-state NMR techniques, confirming conserved secondary structure.

Ultra-High Resolution Solid-State NMR for High Molecular Weight Proteins on GHz-Class Spectrometers

This study improves solid-state NMR resolution for high molecular weight proteins using GHz-class spectrometers and novel techniques, achieving better than 0.2 ppm resolution for proteins up to 144 kDa.

Alpha-Synuclein Fibril Structures Cluster into Distinct Classes

The paper classifies Alpha-synuclein fibril structures into two main polymorph classes using clustering and finds conserved motifs that could guide drug design.

Backbone and Sidechain 1H, 15N and 13C Resonance Assignments of a Multidrug Efflux Membrane Protein using Solution and Solid-State NMR

The paper reports NMR resonance assignments for EmrE, a multidrug transporter, using solution and solid-state NMR techniques at specific pH conditions.

In situ light-driven pH modulation for NMR studies

This paper introduces a method to control pH in NMR samples using light-activated photoacids, enabling non-invasive and precise pH modulation for studying protonation states in proteins.

High-Resolution Cryo-EM Structure Determination of α-Synuclein - A Prototypical Amyloid Fibril

This paper provides a protocol for high-resolution cryo-EM structure determination of α-synuclein fibrils, resulting in a 2.04 Å structure with new density features.

Structure of alpha-synuclein fibrils derived from human Lewy body dementia tissue

This study determines the atomic structure of alpha-synuclein fibrils from Lewy body dementia tissue using a novel amplification method and solid-state NMR.