Katherine A. Henzler‐Wildman

Protonated Structure of EmrE Reveals C-terminal Tail Gating Mechanism

The paper reports the high-resolution structure of protonated EmrE, revealing how the C-terminal tail occludes the transport pore to prevent proton leak.

Discovery of metabolites produced by reactions between central carbon metabolites and cysteine that mark inflammatory macrophages

The paper identifies sulfur-containing metabolites specific to inflammatory macrophages, showing they are produced from cysteine and central metabolism intermediates and are elevated in human inflammatory lesions.

Energetic and structural control of polyspecificity in a multidrug transporter

This study investigates how energy and structure control multidrug transporter specificity by analyzing mutations across substrates and energy conditions.

The C-terminus of the multi-drug efflux pump EmrE prevents proton leak by gating transport

This paper shows that the C-terminal tail of EmrE prevents proton leak by acting as a secondary gate, which is unlocked by substrate binding.

Backbone and Sidechain 1H, 15N and 13C Resonance Assignments of a Multidrug Efflux Membrane Protein using Solution and Solid-State NMR

The paper reports NMR resonance assignments for EmrE, a multidrug transporter, using solution and solid-state NMR techniques at specific pH conditions.

In situ light-driven pH modulation for NMR studies

This paper introduces a method to control pH in NMR samples using light-activated photoacids, enabling non-invasive and precise pH modulation for studying protonation states in proteins.