Thermodynamic Diversity in Staphylococcal Nitrate Sensors with a Conserved Binding Pocket
Elizabeth K. Pack, Ke Feng Ji, Jonathan Martin, Weicheng Peng, Mariah A. Cook, Gabriele Meloni, Sheel C. Dodani
The paper identifies and characterizes nitrate-binding affinity differences in staphylococcal NreA homologues, showing that a conserved binding pocket allows recognition while distal sequence variation tunes affinity.
bioRxiv · Sep 23, 2026 · PreprintBiochemistry