Sjors H. W. Scheres

The paper reports cryo-EM structures of α-synuclein filaments from Parkinson's disease, PD dementia, and dementia with Lewy bodies, showing they are composed of a single protofilament distinct from those in multiple system atrophy.

This study identifies age-dependent formation of TMEM106B amyloid filaments in human brains using cryo-EM, showing they appear in older individuals but not younger ones.

This study reports in vitro assembly of recombinant tau into filaments structurally similar to those in Alzheimer's disease and chronic traumatic encephalopathy using cryo-EM.

Cryo-EM shows brain-derived Aβ42 filaments adopt two related S-shaped protofilament folds: Type I dominates sporadic Alzheimer's, Type II familial Alzheimer's, other disorders and App^NL-F knock-in mice.

Cryo-EM structures sort four-repeat tauopathies into three-layered (PSP, GGT, LNT) and four-layered (CBD, AGD, ARTAG, MAPT intron 10 mutations) tau folds, grounding a hierarchical structure-based classification.

This paper introduces a regularisation framework for cryo-EM structure determination using a convolutional neural network trained on known macromolecular structures, showing improved reconstructions on simulated data.

This study determines the structure of α-synuclein filaments in multiple system atrophy using cryo-EM, revealing distinct filament types and conformers that differ from in vitro assemblies.

This paper reports the structure of tau filaments in corticobasal degeneration using cryo-EM, revealing a novel four-layered fold distinct from other tauopathies.

The paper uses cryo-EM to determine the structure of dynein microtubule-binding domains, revealing structural differences between cytoplasmic and axonemal dyneins that may affect microtubule distortion.

The paper determines the structure of tau filaments in Alzheimer's disease cases and finds they adopt a common fold across individuals.

RELION 2.0 adds empirical Bayesian helical reconstruction with orientation priors, filament-level half-sets and local twist/rise optimisation, yielding near-atomic maps for four filament datasets.