Sofia Lövestam

Templated seeding induces the formation of Alzheimer paired helical tau filaments in human brain organoids

Cortical organoids from healthy human iPSCs, seeded with Alzheimer brain tau, form three-repeat tau filaments that cryo-EM shows are identical to Alzheimer paired helical filaments and need endogenous tau.

Templated paired helical filament formation and tau fold-dependent inclusion morphologies in primary neurons

This study shows that primary neurons can propagate Alzheimer's disease paired helical filaments and that tau filament folds determine inclusion morphologies.

Prion-like transmission of human tau strains in the mouse brain

This study demonstrates that tau filaments from Alzheimer's and corticobasal degeneration patients can seed the formation of structurally identical mouse tau filaments, supporting the prion-like transmission hypothesis.

The Pick fold in tau filaments from human MAPT mutants

This study reports cryo-EM structures of tau filaments from MAPT mutants, showing how specific mutations alter the Pick fold structure and enable reconstitution of variants.

Twelve phosphomimetic mutations induce the assembly of recombinant full-length human tau into paired helical filaments

This study identifies twelve phosphomimetic mutations in tau that induce the formation of paired helical filaments in vitro, mimicking Alzheimer's disease structures.

Cryo-EM image processing of amyloid filaments in RELION-5.1

The paper introduces new tools in RELION-5.1 for automated processing and analysis of cryo-EM images of amyloid filaments, demonstrating their application on experimental datasets.

Seed structure and phosphorylation in the fuzzy coat impact tau seeding competency

This study shows that tau filament seeding capacity depends on both core structure and phosphorylation in the fuzzy coat, with phospho-mimetic residues restoring activity in modified tau.

Serial amplification of tau filaments using Alzheimer's brain homogenates and C322A or C322S recombinant tau

This study investigates tau filament assembly using mutant recombinant tau and brain homogenates, finding that specific mutations produce filaments resembling AD pathology.

Cryo-EM structure of the vault from human brain reveals symmetry mismatch at its caps

The paper presents the cryo-EM structure of vault particles from human brain tissue, revealing a symmetry mismatch at their caps and its implications for therapeutic modifications.

Tau filaments with the Alzheimer fold in cases with MAPT mutations V337M and R406W

The paper determines the structures of tau filaments in individuals with MAPT mutations V337M and R406W using cryo-EM, finding the Alzheimer fold in all cases and a new triple filament assembly.

Cryo-EM structures of tau filaments from SH-SY5Y cells seeded with brain extracts from cases of Alzheimer's disease and corticobasal degeneration

The paper reports cryo-EM structures of tau filaments formed in SH-SY5Y cells seeded with brain extracts from Alzheimer's and corticobasal degeneration cases, showing some structural templating.

Cryo-EM structures of amyloid-beta filaments with the Arctic mutation (E22G) from human and mouse brains

The paper reports cryo-EM structures of amyloid-beta filaments with the Arctic mutation from human and mouse brains, revealing structural differences and conformational changes.

New SNCA mutation and structures of α-synuclein filaments from juvenile-onset synucleinopathy

The paper identifies a SNCA mutation causing juvenile-onset synucleinopathy and describes the structural features of α-synuclein filaments associated with the disease.

Age-dependent formation of TMEM106B amyloid filaments in human brain

This study identifies age-dependent formation of TMEM106B amyloid filaments in human brains using cryo-EM, showing they appear in older individuals but not younger ones.

Assembly of recombinant tau into filaments identical to those of Alzheimer's disease and chronic traumatic encephalopathy

This study reports in vitro assembly of recombinant tau into filaments structurally similar to those in Alzheimer's disease and chronic traumatic encephalopathy using cryo-EM.

Cryo-EM Structures of Amyloid-β 42 Filaments from Human Brain

Cryo-EM shows brain-derived Aβ42 filaments adopt two related S-shaped protofilament folds: Type I dominates sporadic Alzheimer's, Type II familial Alzheimer's, other disorders and App^NL-F knock-in mice.

Seeded assembly in vitro does not replicate the structures of α-synuclein filaments from multiple system atrophy