Overcoming air-water interface induced artifacts in Cryo-EM with protein nanocrates
Matthew C. Jenkins, Daija Bobe, Jake D. Johnston, Jonah Cheung, Akira Karasawa, Christina M. Zimanyi, Ömer Dermanci, Collin T. McManus, M. G. Finn, Alex de Marco, Mykhailo Kopylov
This paper introduces a method to reduce air-water interface artifacts in cryo-EM by encapsulating proteins in hydrophilic shells, demonstrating its effectiveness with three protein examples.
Nature Methods · Aug 24, 2026Peer-reviewedBioengineering