Daija Bobe

Eg5 activity and density-driven bundling organize the human metaphase mitotic spindle independently of spindle bipolarity

The paper shows that Eg5 organizes antiparallel microtubule bundles in human metaphase spindles through density-driven steric interactions, independent of spindle bipolarity.

Overcoming air-water interface induced artifacts in Cryo-EM with protein nanocrates

This paper introduces a method to reduce air-water interface artifacts in cryo-EM by encapsulating proteins in hydrophilic shells, demonstrating its effectiveness with three protein examples.

A robust workflow for 3D imaging of human mitochondria using cryo-electron tomography

The paper presents a workflow for 3D imaging of human mitochondria using cryo-electron tomography to study their structure under different conditions.

Understanding the infection mechanism of {Phi}8 using infection method in Pseudomonas syringae pv. Phaseolicola strain: In-situ CryoET analysis

This study uses Cryo-ET to analyze the infection mechanism of {Phi}8 in Pseudomonas syringae pv. phaseolicola, revealing key stages of viral assembly and extracellular virion localization.

Environmental differences impact Acinetobacter baumannii phage isolation and infectivity

This paper discovers a novel lytic phage, Mystique, that infects a broad range of Acinetobacter baumannii strains and shows that environmental conditions significantly affect phage infectivity and isolation outcomes.

Cryo-ET reveals the in situ architecture of the polar tube invasion apparatus from microsporidian parasites

The paper uses cryo-electron tomography to reveal the in situ architecture of the polar tube invasion apparatus in microsporidian parasites.

Identification and cryoEM structure determination of Escherichia phage YDC107 tail found in a bacteria-contaminated buffer

The paper identifies a contaminant phage from a cryoEM grid and determines its tail structure using ML-based methods without prior sequence knowledge.

Multi-species cryoEM calibration and workflow verification standard

The paper introduces a multi-species cryoEM calibration sample to validate instrument performance and image processing workflows.

Benchmarking cryo-EM single particle analysis workflow

The paper benchmarks cryo-EM single particle analysis workflows using a standard sample and procedures to ensure high-resolution map production.