Mykhailo Kopylov

This paper introduces a pipeline for high-resolution cryo-ET imaging using square electron beams, demonstrating improved resolution and field-of-view by reducing radiation damage and overlap.

This paper introduces a method to reduce air-water interface artifacts in cryo-EM by encapsulating proteins in hydrophilic shells, demonstrating its effectiveness with three protein examples.

This paper introduces a crossed laser phase plate (xLPP) for cryoEM, demonstrating improved contrast and resolution in imaging biological samples.

The paper uses language modeling to create a comprehensive representation of protein biology, enabling structure prediction and discovery of high-affinity protein interactions.

The paper introduces PP7 virus-like particles as a standardized benchmark for cryo-electron tomography, enabling rapid validation and resolution assessment of tomographic pipelines.

This paper introduces tilt-corrected bright-field scanning-TEM (tcBF-STEM) for cryo-EM, showing improved contrast and efficiency for thick samples, with a subnanometer resolution SPA map of a virus-like particle.

The paper presents a cryo-EM conformational heterogeneity challenge to evaluate and improve methods for analyzing molecular motions in heterogeneous samples.

This paper discovers a novel lytic phage, Mystique, that infects a broad range of Acinetobacter baumannii strains and shows that environmental conditions significantly affect phage infectivity and isolation outcomes.

The paper uses cryo-electron tomography to reveal the in situ architecture of the polar tube invasion apparatus in microsporidian parasites.

The paper identifies a contaminant phage from a cryoEM grid and determines its tail structure using ML-based methods without prior sequence knowledge.

The paper introduces a multi-species cryoEM calibration sample to validate instrument performance and image processing workflows.

This paper develops a vaccine strategy using immunologically orthogonal protein nanoparticles to focus antibody responses on a specific SARS-CoV-2 RBD epitope.

This study examines how data compression and lossy image formats affect cryoEM reconstruction quality, finding that compressed data can still yield sub-2Å reconstructions.

The paper benchmarks cryo-EM single particle analysis workflows using a standard sample and procedures to ensure high-resolution map production.