Yuichiro Mishima

Ultra-parallel ribosome profiling platform with RNA-dependent RNA amplification

The paper develops a semi-automated Ribo-Seq and Disome-Seq platform to analyze translation status across many samples, identifying factors regulating translation and revealing links between stop codon readthrough and mitochondrial translation deficiency in COVID-19.

Znf598-mediated Rps10/eS10 ubiquitination contributes to the ribosome ubiquitination dynamics during zebrafish development

The paper investigates ribosome ubiquitination dynamics during zebrafish development, identifying Znf598-mediated ubiquitination of Rps10/eS10 as a key regulatory mechanism.

Translation of zinc finger domains induces ribosome collision and Znf598-dependent mRNA decay in vertebrates

The paper investigates how Znf598-mediated no-go decay (NGD) degrades mRNAs encoding C2H2-ZF domains during zebrafish development, preventing ribosome collisions and maintaining translational homeostasis.

Transient ribosome slowdown at the decoding step triggers mRNA degradation independent of Znf598 in zebrafish

The paper identifies codon-mediated mRNA decay in zebrafish embryos, showing ribosome decoding kinetics affect mRNA stability independently of Znf598 and stall-dependent decay.

Genome-wide survey of ribosome collision

The paper identifies genome-wide ribosome collision sites in humans and zebrafish, revealing their sequence motifs and functional roles in protein synthesis.