Organyl 5'-Phosphates in siRNA Guide Strands: Structure-Function Relationships Governing Anchoring in Argonaute 2 and Metabolic Stability
Theodore Carrigan-Broda, Luca F. R. Gebert, Samuel R. Hildebrand, Nozomi Yamada, Eric Luu, Jillian Caiazzi, Nicholas McHugh, Dimas Echeverria, Atish A. Wagh, Jonathan K. Watts, Anastasia Khvorova, Ian J. MacRae, Ken Yamada
This study identifies organyl 5'-phosphate mimics that enhance AGO2 binding and metabolic stability by interacting with a hydrophobic pocket in the MID domain.
bioRxiv · Feb 14, 2026 · PreprintMolecular Biology