Emilia A. Zin

Viability of engineered AAVs via protein language models

The paper uses AI to analyze the structural impact of 7-mer peptide insertions in AAV capsids, identifying viable modifications and their effects on capsid stability and function.

Promfusion: a synthetic fusion promoter enabling enhanced and balanced photoreceptor transgene expression

The paper develops and evaluates two synthetic fusion promoters, Pikali and Nocchu, for enhanced and balanced transgene expression in both rods and cones of retinal organoids.

Optimal sequencing depth for measuring the concentrations of molecular barcodes

The paper determines the optimal sequencing depth for accurately measuring barcode concentrations in combinatorial genetic experiments by analyzing NGS data and incorporating a mathematical model of PCR amplification.

The Role of Thermal Stability in AAV Titration of Engineered Variants

The paper investigates why engineered AAV variants have lower titers and finds that DNAse digestion heat reduces their stability, leading to artificially low titers.

Uncursing winner's curse: on-line monitoring of directed evolution convergence

The paper introduces ACIDES, a method that improves performance estimation in directed evolution by combining statistical inference and in-silico simulations, tested on experimental and public datasets.

Outcomes of Progranulin Gene Therapy in the Retina are Dependent on Time of Delivery

This study shows that progranulin gene therapy in a CLN11 mouse model is time-sensitive, with early administration reducing retinal thinning but no benefit at later ages.