Molecular BiologybioRxivPreprint
✦ In-depth digest
An integrated pipeline to count individual transcripts with single-cell resolution
Eva Sheardown, Jane Yan To Ling, Servaas N. van der Burght…·QueeLim Ch'ng
HCR3.0 RNA-FISH linked to RS-FISH spot detection labels transcripts in C. elegans embryos, larvae and adults, and yields per-neuron counts of short insulin-like peptide mRNAs in L2 larvae.
DOI 10.64898/2026.04.15.718387