Servaas N. van der Burght

HCR3.0 RNA-FISH linked to RS-FISH spot detection labels transcripts in C. elegans embryos, larvae and adults, and yields per-neuron counts of short insulin-like peptide mRNAs in L2 larvae.

This study constructs a lineage-resolved single-cell multimodal atlas of early C. elegans development, revealing genomic dynamics and zygotic genome activation mechanisms.

The paper identifies a mechanism by which C. elegans ASE neurons maintain their identity despite molecular fluctuations through the reservoir effect of the transcription factor CHE-1.