Author

Melissa A. Chiasson

University of WashingtonORCID 0000-0002-1880-3181Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Organism:human· 3 Approach:experimental assay· 2 Assay:proteomics· 2 Research Focus:variant effect prediction· 2 Approach:statistical model· 1

Frequent Co-Authors

Douglas M. FowleriD· 3 shared Allan E. RettieiD· 2 shared Jason J. Stephany· 2 shared Katherine A. SitkoiD· 2 shared Kenneth A. MatreyekiD· 2 shared Arineh Khechaduri· 1 shared Beth MartiniD· 1 shared Clara J. AmorosiiD· 1 shared

Activity by Year

2018-2021 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 31 mutual co-author links

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3 shared papersGenomics

Douglas M. FowlerORCID

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Author profile →

3 papers

Recent·Most cited

Massively parallel characterization of CYP2C9 variant enzyme activity and abundance

Clara J. Amorosi, Melissa A. Chiasson, Matthew G. McDonald, Lai Hong Wong, Katherine A. Sitko, Gabriel E. Boyle, John P. Kowalski, Allan E. Rettie, Douglas M. Fowler, Maitreya J. Dunham

The paper characterizes CYP2C9 variant enzyme activity and abundance using Click-seq and VAMP-seq, finding that two-thirds of variants show decreased activity and abundance explains half the functional variation.

The American Journal of Human Genetics · Jul 26, 2021Peer-reviewedGenomics

Multiplexed measurement of variant abundance and activity reveals VKOR topology, active site and human variant impact

Melissa A. Chiasson, Nathan J. Rollins, Jason J. Stephany, Katherine A. Sitko, Kenneth A. Matreyek, Marta Verby, Song Sun, Frederick P. Roth, Daniel DeSloover, Debora S. Marks, Allan E. Rettie, Douglas M. Fowler

The paper measures the effects of VKOR variants on abundance and activity in human cells, revealing its topology, active site, and the impact of human variants.

eLife · Sep 1, 2020Peer-reviewedGenomics

Multiplex Assessment of Protein Variant Abundance by Massively Parallel Sequencing

Kenneth A. Matreyek, Lea M. Starita, Jason J. Stephany, Beth Martin, Melissa A. Chiasson, Vanessa E. Gray, Martin Kircher, Arineh Khechaduri, Jennifer N. Dines, Ronald J. Hause, Smita Bhatia, William Edward Evans, Mary V. Relling, Wenjian Yang, Jay Shendure, Douglas M. Fowler

This paper introduces VAMP-seq, a method to assess the abundance of protein variants, and identifies pathogenic variants in PTEN and TPMT.

Nature Genetics · May 20, 2018Peer-reviewedGenetics