Synthetic auxotrophy remains stable after continuous evolution and in co-culture with mammalian cells
Aditya M. Kunjapur, Michael G. Napolitano, Eriona Hysolli, Karen Noguera, Evan Appleton, Max G. Schubert, Michaela A. Jones, Siddharth Iyer, Daniel J. Mandell, George McDonald Church
The paper continuously evolves a synthetic auxotrophic E. coli strain and finds it remains contained over 100 days and in co-culture with mammalian cells.
Science Advances · Jul 2, 2021Peer-reviewedSynthetic Biology