Daniel Bryan Goodman

The paper uses CRISPR to assess the functional impact of IL2RG variants in human T cells, confirming known annotations and identifying novel loss-of-function mutations.

This paper develops a method to efficiently reprogram T cells at multiple loci using SEED-Selection, achieving high purity for single and combined gene edits.

A 240-species placental mammal alignment resolves constraint to single bases, estimating at least 10.7% of the human genome is constrained, with 48.5% of constrained bases unannotated.

This paper introduces RLR, a high-throughput method for screening genetic variants using in-vivo single-stranded DNA production, demonstrating its utility in antibiotic resistance studies.

The paper identifies rare genetic variants that cause large disruptions to exon recognition, using a new assay called MFASS, and finds most of these variants are not in canonical splice sites.

The paper presents a method combining multiplex genome engineering and predictive modeling to identify minimal genomic modifications that optimize a complex phenotype in E. coli.

Millstone is a web-based platform for microbial genome analysis and engineering that automates genotype comparison and visualization for projects with up to hundreds of genomic samples.