Evan Appleton

DERIVATION OF ELEPHANT INDUCED PLURIPOTENT STEM CELLS

This paper reports the first derivation of elephant induced pluripotent stem cells (emiPSCs) using a modified reprogramming protocol that inhibits TP53 expansion genes.

Simulating a for-loop in the human genome: Design and evaluation of recombinase genetic programs that count to three.

The paper designs and evaluates recombinase-based genetic programs in human cells that simulate a for-loop counting to three, demonstrating heritable memory and sequential gene expression.

Machine-guided cell-fate engineering

The paper introduces a machine-learning pipeline, CellCartographer, to design TF pooled-screens for cell type conversion using chromatin accessibility data, achieving efficient differentiation of iPSCs into six cell types within 6 days.

helixCAM: A Platform for Programmable Cellular Assembly in Bacteria and Human Cells

The paper introduces helixCAM, a platform for engineering specific cell-cell interactions in bacteria and human cells using coiled-coil peptides, enabling multicellular assembly applications.

Synthetic auxotrophy remains stable after continuous evolution and in co-culture with mammalian cells

The paper continuously evolves a synthetic auxotrophic E. coli strain and finds it remains contained over 100 days and in co-culture with mammalian cells.

Algorithms for the selection of fluorescent reporters

The paper presents algorithms and an open-source tool to optimize fluorophore panels for maximal signal and minimal bleed-through in fluorescent measurements.

Genetic design automation for autonomous formation of multicellular shapes from a single cell progenitor

The paper introduces a computational method to design genetic circuits that control the formation of multicellular shapes from a single cell in human cells.