This study investigates the metal ion activation and DNA recognition mechanisms of DR2539 in Deinococcus radiodurans using biophysical analyses and structural data.

FEBS Journal · Apr 23, 2024Peer-reviewedBiophysics

Crk proteins activate the Rap1 guanine nucleotide exchange factor C3G by segregated adaptor-dependent and -independent mechanisms

Antonio Rodríguez-Blázquez, Arturo Carabias, Alba Morán-Vaquero, Sergio de Cima, Juan Román Luque-Ortega, Carlos Alfonso, Peter Schuck, José A. Manso, Sandra Macedo‐Ribeiro, Carmen Guerrero, José M. de Pereda

This study identifies two distinct mechanisms by which Crk proteins activate C3G: adaptor-dependent interactions with specific motifs and phosphorylation-dependent priming.

Cell Communication and Signaling · Feb 3, 2023Peer-reviewedMolecular Biology

Critical Assessment of Protein Intrinsic Disorder Prediction

Marco Necci, Damiano Piovesan, CAID Predictors, Md Tamjidul Hoque, Ian Walsh, Sumaiya Iqbal, Michele Vendruscolo, Pietro Sormanni, Chen Wang, Daniele Raimondi, Ronesh Sharma, Yaoqi Zhou, Thomas Litfin, Oxana V. Galzitskaya, Michail Yu. Lobanov, , Silvio C. E. Tosatto

The paper evaluates 43 methods for predicting intrinsically disordered regions and binding regions in proteins, finding that deep learning methods outperform older physicochemical approaches but binding prediction remains challenging.

Nature Methods · Apr 19, 2021Peer-reviewedBioinformatics