Steven R. Shuken

This study characterizes axolotl perilymph-cerebrospinal fluid (P-CSF) during limb regeneration, identifying proteomic and metabolomic changes associated with amputation.

This paper introduces a high-resolution multi-pass Astral analyzer mode for TMT proteomics, enabling 35-plex quantification with improved depth and precision.

GoDig-LiF enables targeted proteomics without data libraries by using predicted spectra and retention times, applied to quantify mutated proteins in cancer cell lines.

GoDig 2.0 improves targeted proteomics by increasing multiplexing, efficiency, and target coverage, enabling analysis of post-translational modifications and compound-protein interactions.

The paper identifies CD72 as a regulator of microglial tiling through proteomic profiling and functional assays.

The paper identifies a microglia population that takes up circulatory proteins, enhancing phagocytosis and innate immunity through ApoA-I uptake.

The paper introduces GoDigViewer to optimize GoDig assays by implementing chromatographic priming runs, significantly improving peptide-level quantification success rates in targeted pathway proteomics.

The paper models and visualizes the costs and benefits of p-value correction for multiple hypothesis testing in omics data using simulated experiments and an interactive R Shiny application.