Cedric K. Brinkmann

The paper develops a chloroplast-based cell-free gene expression platform across multiple plant species to accelerate synthetic biology research and gene expression studies.

This study establishes Chlamydomonas reinhardtii as a platform for chloroplast synthetic biology, enabling high-throughput engineering of plastomes and demonstrating improved biomass production through synthetic pathways.

The paper demonstrates the use of L-SCRaMbLE to generate large-scale genome rearrangements in synthetic Sc2.0 yeast chromosomes through light-inducible Cre recombinase.