María del Carmen Sánchez Olmos

This paper presents a coupled strategy for enzyme discovery, evolution, and yeast chassis adaptation to improve microbial biopolymer valorisation, demonstrating it with laccases for lignin depolymerisation.

The paper demonstrates the use of L-SCRaMbLE to generate large-scale genome rearrangements in synthetic Sc2.0 yeast chromosomes through light-inducible Cre recombinase.

A de novo yeast neochromosome carrying all 275 tRNA genes was built and characterized; it imposes a growth burden, with genome doubling and deletions in haploids possibly reflecting compensation.