Daniel P. Gitterman

SELECT-seq enables rapid, scalable SNP-specific enrichment of single cells for genotype-phenotype analysis without cloning.

Saturation-seq combines genome editing and single-cell RNA-seq to quantify variant effects on NFE2L2, accurately distinguishing pathogenic from benign variants.

ONE-STEP tagging enables rapid, efficient, and scalable protein tagging in diverse cell types through CRISPR-Cas9 and Bxb1 integrase, with high integration efficiency and multiplexing capabilities.

The paper uses saturation genome editing to assess DDX3X variants' functional impact, identifies pathogenic variants for neurodevelopmental disorders, and develops a classifier outperforming existing tools.