Sho Iwama

STEER-seq enables pooled single-cell perturbation screening in hiPSCs by using defined genomic integration and barcode tracking to study transcription factor effects on cell identity.

SELECT-seq enables rapid, scalable SNP-specific enrichment of single cells for genotype-phenotype analysis without cloning.

The paper validates an Alzheimer's disease-associated SNP in PTK2B that alters microglial function through changes in chromatin accessibility and gene expression.

This paper develops a pooled paired prime editing screen to generate large-scale deletions across the genome, identifying non-coding regions with fitness effects and demonstrating the feasibility of genome-wide deletion perturbation.

ONE-STEP tagging enables rapid, efficient, and scalable protein tagging in diverse cell types through CRISPR-Cas9 and Bxb1 integrase, with high integration efficiency and multiplexing capabilities.