Wandong Bao

This study generates genetically identical mammalian oocytes using parthenogenetic double-haploid embryonic stem cells and blastocyst complementation, producing viable and fertile mice with epigenetic defects.

The paper shows that efficient reprogramming in SCNT occurs exclusively in the epiblast, enabling cloned mouse generation through a lineage-specific mechanism.

This study derives and characterizes mouse extra-embryonic endoderm stem cell lines from somatic cell nuclear transfer, showing high efficiency and distinct gene expression profiles compared to fertilization-derived lines.