Skevoulla Christou

This study shows that combining dCas9 and Cas9 improves viability and reduces off-target effects when editing genes linked to viability or paralogous clusters.

The paper analyzes 3,313 genetically engineered mouse lines to identify gene essentiality as the main factor affecting Cas9-based genome editing success.

This study uses long-read sequencing to accurately identify correct alleles in genome-edited animals despite mosaicism and complex edits.

The paper reports the generation of over 5,000 new mouse mutant strains for targeted genes, significantly expanding the available genetic resources for biomedical research.