Author

Alessandro Migliara

The San Raffaele Telethon Institute for Gene TherapyORCID 0000-0002-3979-3891Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Synthetic Biology· 2

Signature Assays, Organisms & Methods

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Assay:CRISPR screen· 2 Approach:experimental assay· 2 Organism:human· 2 Assay:ATAC-seq· 1 Research Focus:benchmarking· 1 Research Focus:cell type annotation· 1 Research Focus:gene regulatory network inference· 1

Frequent Co-Authors

Angelo LombardoiD· 1 shared Benoit G. BruneauiD· 1 shared Davide CittaroiD· 1 shared Ivan MerelliiD· 1 shared Martino Alfredo CappellutiiD· 1 shared Oscar A. CamposiD· 1 shared Pilar Lopez· 1 shared Ricardo AlmeidaiD· 1 shared

Activity by Year

2022-2025 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 20 mutual co-author links

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1 shared paperSynthetic Biology

Angelo LombardoORCID

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2 papers

Recent·Most cited

Engineering Synthetic Cell State Transitions by Harnessing Epigenetic Memory

Oscar A. Campos, Alessandro Migliara, Sarah R Wasinger, Zhe Chen, Zoe L. Grant, Pilar Lopez, Benoit G. Bruneau, Satoshi Toda, Wendell A. Lim, Ricardo Almeida

This paper engineers synthetic epigenetic circuits that induce stable gene expression changes and drive cell state transitions through transient cell-cell interactions.

bioRxiv · Sep 29, 2025 · PreprintSynthetic Biology

In vitro selection of Engineered Transcriptional Repressors for targeted epigenetic silencing and initial evaluation of their specificity profile

Alessandro Migliara, Martino Alfredo Cappelluti, Sara Valsoni, Ivan Merelli, Davide Cittaro, Angelo Lombardo

The paper describes an in vitro screening protocol to identify effective gRNAs for epigenetic silencing using engineered transcriptional repressors and evaluates their genome-wide specificity.

bioRxiv · Aug 30, 2022 · PreprintSynthetic Biology