Wendell A. Lim

The paper designs synthetic scaffold proteins that form cell-cell interfaces with switch-like condensation behaviors, enabling selective and specific intercellular interactions.

This study engineers orthogonal chemokine-receptor pairs to enable targeted immune cell migration and collective swarming behavior in engineered T cells.

The paper shows coupling reaction-diffusion circuits with mechanical regulation improves robustness of self-organized pattern formation in synthetic cell systems.

This paper identifies methods to achieve ultra-large DNA integrations (>10 kb) in human cells with high efficiency using optimized templates and delivery strategies.

This paper develops a spike-in probe-enhanced scRNA-seq method to track and profile synNotch-CAR-T cells in vivo, revealing tissue-specific activation and transcriptional states.

This study shows that combining differential adhesion with cell signaling avoids kinetic traps, enabling robust multicellular self-organization in synthetic systems.

This paper engineers synthetic epigenetic circuits that induce stable gene expression changes and drive cell state transitions through transient cell-cell interactions.

This study uses programmable microparticles to enhance CAR-T cell expansion beyond physiological limits by rewiring signaling pathways.

This paper engineers T cells with synthetic receptors that activate only in the lung, demonstrating RAGE as a lung-specific target for targeted therapies.

This paper introduces bioPROTACs, a genetic tool to control CAR T cell signaling by degrading specific proteins, demonstrating its effectiveness in inhibiting CAR signaling in human T cells.

This study demonstrates that de novo-designed minibinders can be adapted to various cell engineering tools, improving antigen detection and cytotoxicity compared to antibody-based systems.

The paper uses synthetic costimulatory domains and machine learning to identify design rules for CARs that enhance cytotoxicity and stemness.

This paper engineers T cells with a two-step positive feedback circuit to kill target cells based on an ultrasensitive antigen density threshold.

The paper identifies five core network motifs capable of temporal filtering and shows how combinations of two motifs underlie natural timer circuits in cellular processes.