Grant T. Daly

The paper uses MOA-seq to map transcription factor occupancy in hypoxia-treated human endothelial cells, identifying thousands of hypoxia-responsive sites and clustering them into HIF1A-associated and independent TF groups.

The paper identifies thousands of alternative splicing events in human vascular endothelial cells under hypoxia, revealing a more complex and dynamic transcriptional response than conventional RNA-seq suggests.

The paper presents a computational method to generate synthetic eukaryotic signal peptides using building blocks, validated by Signal-BLAST showing 88% of generated sequences were identified as signal peptides.