Vittoria Raffa

The extremely low mechanical force generated by nano-pulling induces global changes in the microtubule network, nuclear morphology, and chromatin transcription in neurons

A Transfection-Free Approach of Gene Editing via a gold-based nanoformulation of the Cas9 protein

This paper presents a transfection-free method for delivering Cas9:gRNA ribonucleoprotein using gold nanoparticles, achieving effective gene editing in human melanoma cells.

A photo-switchable gold nanoformulation based on the dCas9 protein for spatiotemporal controlled gene editing activation in vivo

This paper introduces a nano-switch using dCas9 and gold nanorods to enable spatiotemporal control of gene editing in zebrafish embryos through near-infrared laser activation.

Long-term mouse spinal cord organotypic slice culture as a platform for validating cell transplantation in spinal cord injury

This paper describes an optimized protocol for long-term maintenance of mouse spinal cord organotypic slices transplanted with human neural stem cells to test cell replacement therapies for spinal cord injury.

Nano-pulling stimulates axon regeneration in dorsal root ganglia by inducing stabilization of axonal microtubules and activation of local translation

The paper demonstrates that nano-pulling enhances axon regeneration in dorsal root ganglia by stabilizing microtubules and activating local translation.

Low forces push the maturation of neural precursors into neurons

The paper shows that mechanical stimulation via magnetic nano-pulling accelerates neural precursor maturation into neurons and alters neuronal network organization.

Axonal plasticity in response to active forces generated through magnetic nano-pulling

This study shows that magnetic nano-pulling induces axonal cytoskeleton remodeling, enhancing outgrowth and synapse maturation through microtubule density increase and local translation promotion.